In order to evaluate the effect of raw enzymatic extract on the marigold petals, the supernatants obtained from mixed culture filtrated were blended with dried or fresh petals in 1:12 (w/v) ratio and kept on rotary shaker at 28 C and 175 rpm (Forma Scientific, model 4520) for 24 h. The samples treated were filtered to separate their solid and liquid phases. The solid phases, marigold petals treated, were processed to obtain marigold flower flour.