When the growth was optimal, they were suspended in sterile saline solution and standardized to 0.5 McFarland density (108 colony-formingunits/ mL (CFU mL1) to achieve confluent growth.
When thegrowth was optimal, they were suspended in sterile saline solutionand standardized to 0.5 McFarland density (108 colony-formingunits/mL (CFU mL1) to achieve confluent growth.