Effects of Sucrose
The highest regeneration percentage (93.19) was found
in the medium with 90 g/L sucrose. It was observed that
regeneration percentage increased up to 90g/L of sucrose
and then it was decreased. The earliest (36.95 days)
bulblets induction was observed at 90g/L sucrose but it
was delayed either increasing or decreasing of sucrose
level. The highest (1.79) number and heaviest (2.17 g)
weight of bulb was also achieved at 90 g/L sucrose level.
Percent of undesirable shooting decreased significantly
with increasing sucrose level up to 90 g/L. It appeared that
medium supplemented with 90 g/L sucrose was suitable
for achieving maximum number of bulbs per twin scales
and was used for all further studies in combination with
BAP and CCC.