The melanocortin-5 receptor (MC5R) gene is a key factor in the regulation of exocrine gland function which controls gastric juice production and cooperates with enzyme from pancreas. In this study, the genetic polymorphisms of MC5R genes in 220 indigenous chickens (Praduhangdum) were identified. The associations of MC5R genes with growth Rate in indigenous chickens were studied. Three single nucleotide polymorphisms (SNPs) in the chicken MC5R genes were found in 5’-flanking regions at the position of 78(A>G), 249(A>G), and 388(T>A). One position of SNPs was developed as the simple DNA marker for genotyping. The Hin6I Restriction enzyme was used to detect SNPs at the position of 249(A>G). The allele frequencies were 0.52 for A and 0.48 for G. The results indicated that the polymorphisms of MC5R markers associated with average daily gain.It showed that average daily gain in indigenous chickens with genotype GG was higher than that of AA (P