MATERIALS AND METHODS
Virus and DNA clones
TYLCV was purified from infected tomato tissues and viral single strandednDNA was extracted from virua particles. Circular double stranded DNA was synthesized and cloned into Bluescript plasmid at EcoRI site. Full length cloned DNAs of TYLCV components A and B were generated and designed as pTYLCA and pTYLCB (Attathom et al.,1990.Chiemsombat et al., 1990).