Soil samples were collected from plastic dump yard in Thiruvananthapuram
(082901500N 76570900E on the west coast). Samples
were collected 5 cm below the soil surface in a sterile
container. Enrichment culture method was followed in which
10 gm of soil sample was transferred into a conical flask with mineral
medium (95 ml), 0.85% NaCl and HIPS film as the sole carbon
source (Cheng et al., 2010). Incubation was done for 14 days at
30 C at 150 rpm. 5 ml of the culture supernatant was transferred
to 45 ml fresh mineral medium with plastic. Further, plating was
done on mineral medium to obtain pure cultures.