All analyses were performed on Shimadzu GC-2010 Plus with
AOC-20i autosampler (Kyoto, Japan) and operated by a computer
running GC solution version 2.41.00 SU1 (Kyoto, Japan). The chromatograph was equipped with Rtx-5 fused silica column
(30 m0.25 mm ID, 0.50μmfilm thickness, Restek, USA) coated
with 5% diphenyl dimethyl polysiloxane. Hydrogen (purity
99.9992%) was employed as carrier gas. The oven temperature
was held at 701C for 0 min following injection and then raised to
3001Cat201C min
1
, total run time was 13 min. Instrument
parameters were as follows: 2501C inlet temperature, 3101C
detector temperature, columnflow 2.82 mL min
1
. All injections
were done in the split mode (1:50) and the volume of injected
sample was 1μL.
Micro-shaker type 326 m was from Premed (Warsaw, Poland)
and ultrasonic bath type Transsonic T 310 was from Elma
(Germany).
All analyses were performed on Shimadzu GC-2010 Plus with
AOC-20i autosampler (Kyoto, Japan) and operated by a computer
running GC solution version 2.41.00 SU1 (Kyoto, Japan). The chromatograph was equipped with Rtx-5 fused silica column
(30 m0.25 mm ID, 0.50μmfilm thickness, Restek, USA) coated
with 5% diphenyl dimethyl polysiloxane. Hydrogen (purity
99.9992%) was employed as carrier gas. The oven temperature
was held at 701C for 0 min following injection and then raised to
3001Cat201C min
1
, total run time was 13 min. Instrument
parameters were as follows: 2501C inlet temperature, 3101C
detector temperature, columnflow 2.82 mL min
1
. All injections
were done in the split mode (1:50) and the volume of injected
sample was 1μL.
Micro-shaker type 326 m was from Premed (Warsaw, Poland)
and ultrasonic bath type Transsonic T 310 was from Elma
(Germany).
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