Materials and Methods Chrysanthemum shoots were collected from the plants grown in the University of Peshawar. Nodal explants were taken from somewhat hard lower portions of the plants. After removing the leaves, stem pieces were washed with household detergent to remove the impurities and then wased thoroughly under tap water.Further sterilization was done with 70% alcohol for 2 minutes followed by 1 % HgCl for 2-3 minutes. Nodal cuttings were finally washed with sterilized distilled water for several time.