The recombinant plasmid, pET28a-gGILT, was transformed into competent BL21 (DE3) (Novagen) cells to express fusion protein. The target protein was purified with His-Bind Columns (Qiagen, Germany) as described previously
The recombinant plasmid, pET28a-gGILT, was transformedinto competent BL21 (DE3) (Novagen) cells to express fusion protein. The target protein was purified with His-Bind Columns (Qiagen, Germany) as described previously