The morning after eggs were laid, 2 randomly selected egg
capsules from the egg cluster were added to each of 6 containers
per trial (3 containers per CO2 concentration per trial). This
balanced the need for numerous embryos with the feasibility of
measuring paralarvae immediately after hatching. An additional 4
containers were included in the water bath as blanks (2 per CO2
concentration) and contained no eggs throughout the experiment
for comparative purposes so that seawater chemistry measurements
could be taken independent of potential biotic effects on
chemistry parameters.