Extraction and recovery of lactate and acetate were validated.
Percentage recovery was determined by adding known concentrations of standards to RTE meat samples, and the samples analyzed using the method described above. Duplicate non-spiked
samples were analyzed to quantify the background concentrations of lactate and acetate. Recovery percentage was calculated by the formula: (amount of analyte recovered)/(amount of analyte
added þ background analyte amount)*100%. The average recovery percentage of lactate and acetate was 91.81 ± 5.50% and 92.64 ± 6.80%, respectively.