When oleic acid was used,Tween 80 was added as emulsifier at 10% (wt/wt) against the fatcontent. All experiments were carried out in 250 mL Erlenmeyerflasks containing 100 mL YPD medium or lipid production mediuminoculated with the pre-culture (initial OD600= 0.01) and incubatedin a rotary shaker at an agitation rate of 220 rpm and incubation temperature of 30◦C.