The main components of the bacterial communities thriving in
rye sourdoughs as well as their dynamics were investigated
through a culture-independent approach by means of DGGE of
amplicons of the V3 region of the 16S rRNA gene (16S rRNAPCR-
DGGE), followed by purification and sequencing of the DGGE
bands, as described before (Van der Meulen et al., 2007).