Antimicrobial secondary metabolites from extremophiles play a significant role in the
pharmacological industry due to their stable and strong activity and it is used in the treatment of
microbial infections. In the present work, Halomonas salifodinae MPM-TC (M. Peter Marian-T.
Citarasu) was isolated from the solar salt works in India and identified by 16S rRNA sequencing.
The secondary metabolites were extracted from H. salifodinae MPM-TC and tested for antibacterial
activity against aquatic bacterial pathogens such as Vibrio harveyi, Vibrio parahaemolyticus, Pseudomonas
aeruginosa and Aeromonas hydrophila isolated from infected fish/shrimp, and it effectively
controlled them with more than 10 mm of zone of inhibition. The metabolites were purified through
silica column chromatography and in vitro antiviral activity was performed against White Spot Syndrome
Virus (WSSV) using different fractions. Among the different tested fractions, fraction-III (FIII)
was able to suppress WSSV replication.