2.4. DNA pools
Association between phenotypes and microsatellite markers was studied using DNA pools of specific phenotypes from the segregating F1 family. Six DNA pools were generated from the following phenotypes: Transparent (TP), Kohaku with low coverage of red (RL), completely red (CR), Bekko with high (BH) and with low (BL) coverage of black and บtsuri (บT). DNA pools were prepared by mixing equal amounts of DNA from each of the 23-25 individual samples to a total concentration of 50 ng/|il, from which 2 |il were used as template in the PCR reaction.