4. Conclusions
A rapid, reliable and sensitive HPLC–MS/MS analytical method
was developed and validated for the quantification of Leu, Ile
and Val in Beagle dog plasma. This method has been successfully
applied to a pharmacokinetic study following oral administration
of an ascending single dose and multiple doses of BCAAs. For the
first time we identified the pharmacokinetic profiles of BCAAs,
and provided theoretical basis for clinical medication and further
assessment of their efficacy and safety