. The open reading frame of gene consisted of 1902 bases. The PCR product was digested in vitro with a non- cutter (HindIII) and cutter (EcoRII) endonuclease as revealed by in silico digestion (web cutter 2.0; http://bio.biomedicine.gu.se/ cutter2) of previously reported cry2A type sequences and our newly obtained cry2A gene sequence.