Both naturally occurring eye color strains of L. lineolaris previously identified were characterized as autosomal recessive alleles. The wild collected eye color variant [52] was reported to have little effect on behavior or physiology, although because of the low field prevalence of the phenotype it was implied that there could be a fitness disadvantage, possibly associated with mating disadvantage based on decreased visual ability. The red-eyed “R” strain was kept in culture for >5 years, but has since been discarded (G. Snodgrass, personal com-munication). The eye color mutation identified through inbreeding [53] was not tested for physiological characteristics, but was observed to display no obvious behavioral or developmental differences from wild type. The strain reported here and the progenitor wild type strain both exhibited apparent loss of fitness. The 20 day cumulative egg production reported for the wild caught red strain was over 120 eggs/female, while the specimens reported here produced less than half that in every cross (Table 1). The loss of fecundity is most distinct in the fecundity of the Ca × W cross. The reason for this loss of fecundity is not known. Interestingly, the total number of male vs. female wild type offspring produced by the Ca × F1 crosses exceeded the critical value for the expected m/f ratio (Table 2A), while the overall phenotype distribution fit the expected results (Table 2B). Inbreeding depression is an accepted phenomenon, relevant to evo-lution, conservation, and agriculture. Intentional in-breeding is also expected to play a vital and dominant role in future molecular genetics and functional genomic research [67].
Both naturally occurring eye color strains of L. lineolaris previously identified were characterized as autosomal recessive alleles. The wild collected eye color variant [52] was reported to have little effect on behavior or physiology, although because of the low field prevalence of the phenotype it was implied that there could be a fitness disadvantage, possibly associated with mating disadvantage based on decreased visual ability. The red-eyed “R” strain was kept in culture for >5 years, but has since been discarded (G. Snodgrass, personal com-munication). The eye color mutation identified through inbreeding [53] was not tested for physiological characteristics, but was observed to display no obvious behavioral or developmental differences from wild type. The strain reported here and the progenitor wild type strain both exhibited apparent loss of fitness. The 20 day cumulative egg production reported for the wild caught red strain was over 120 eggs/female, while the specimens reported here produced less than half that in every cross (Table 1). The loss of fecundity is most distinct in the fecundity of the Ca × W cross. The reason for this loss of fecundity is not known. Interestingly, the total number of male vs. female wild type offspring produced by the Ca × F1 crosses exceeded the critical value for the expected m/f ratio (Table 2A), while the overall phenotype distribution fit the expected results (Table 2B). Inbreeding depression is an accepted phenomenon, relevant to evo-lution, conservation, and agriculture. Intentional in-breeding is also expected to play a vital and dominant role in future molecular genetics and functional genomic research [67].
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