The density of cell suspensions was adjusted to 5% of settled cell volume (SCV) by dilution of 1mL of settled cells into 19 mL of fresh medium. One milliliter of the diluted culture was placed on a filter paper in a Petridish containing 35 mL of culture medium. Five different culture media were used (Table 1). Four treatments were tested, with different use sequences of culture medium for each phase of the regeneration process (differentiation, embryo maturation, germination and