extraction abilities. Prior to the extraction ability
measurement, leachate samples were filtered through
0.45 μm membrane filter (Whatman) and defined as
DOC. Mine tailings were suspended in each leachate
samples at a (w/v) ratio of 1:20 (1 g tailings to 20 ml
leachate) and shaken for 24 hours in a shaker at 200
rpm at room temperature.(12) After that, suspensions
were centrifuged in polypropylene tubes at 10,000
rpm with 20 cm radius of the rotar for 10 minutes in