All spectra replicates of each samples at T0 were employed for
the creation of the Main Spectrum Profile (MSP), by standard MSP
creation method (FlexAnalysis software, Bruker Daltonik) after
preprocessing analysis consisting of mass adjustment, smoothing,
evaluation of substrate baseline, normalization and peak picking.
Final MSP spectra of 3 samples (OPBG_4_T15, OPBG_6_T3,
OPBG_12_T4) were assigned to the correct taxon node of the tax-
onomy tree into the MALDI-TOF MS (MALDI Biotyper 3.0, Bruker
Daltonik) library to custom the spectra library and to perform
analytical ID of other Acanthamoeba isolates.