It is possible to detect internucleosomal DNA fragmentation
by ELISA both in cell culture and human serum/
plasma. In addition to DNA fragmentation, caspasecleaved
cytokeratin 18, also named M30 antigen, which
is a novel biomarker for apoptosis, in either cell culture
or plasma/serum is measured by ELISA (Apoptosense
ELISA assay, Peviva, Sweden). The detection of M30
antigen is particularly convenient and reliable to measure
apoptosis quantitatively in any suitable human sample
(Figure 9). M30 antigen detection by ELISA is reported
to be useful in the management of cancer patients
[28-32) and as a diagnostic tool for one of the common
liver diseases (NASH) [33].