recovery from LN was achieved by quick thawing at 40 C, effectively avoiding ice crystal growth which may occur during warming. This is particularly crucial where somatic embryos, which contain high levels of water, are used use as explant. The present study does not employ any cryoprotectants like dimethyl sulfoxide. reported that use of DMSO for the cryopreservation of Musa seeds resulted in 50% less viability compared to the results obtained when DMSO was not used.