For enumeration of total aerobic counts (TAC)
an aliquot of 100 μl of each of the 10-fold dilutions was spread plated
on plate count agar (Oxoid code, CM0463, Oxoid) and incubated at
30 °C for 48 h. LAB were enumerated by pour plating on DeMan, Rogosa,
Sharpe (MRS) agar (Oxoid code CM1153) and incubating at 30 °C
for 72 h. Pseudomonas spp.