As a restriction of the study, functional T cell tests (i.e.,stimulation with ZIKV antigen followed by intracellular
cytokine staining) have not been performed. Future studies will address lymphocyte and cytokine responses in more detail, allowing proper conclusions of Th1- and Th2-biased cell responses. Despite showing rather preliminary data, to our knowledge, this is the first study investigating immune parameters in acute ZF.