Soluble protein content in the filtrate was
determined according to Bradford (1976) with bovine serum
albumin (Sigma-Aldrich, Germany) as a standard. Protein
leakage was expressed as mg g–1 fresh weight of mycelia.
For assaying lipid peroxidation, a method based on the
reaction of thiobarbituric acid with MDA derived from lipid
peroxidation was employed. Detection of thiobarbituric acid
species was carried out by a colorimetric assay (Ritter et al.
2008). The lipid peroxidation was expressed as μg g–1 fresh
weight of mycelia.