Acclimatization of Bauhinia species
According to Singh et al. (2012), shoots that form
in vitro and that have rooted well can be trimmed
to plantlets containing 3-4 nodes 2-3 cm in size
and the plantlets can be rooted in a mixture of 1 : 1
sand and soil (based on Kumar 1992) with fungicides
added. For rooting, the plantlets were kept at
high humidity for 2 weeks. Watering with 1/10-strength MS medium was sufficient for the survival
of B. variegata plantlets in soil (Mathur and
Mukunthakumar 1992). Thonnalak (2012) showed
64% plantlet survival after transfer to peat moss in
the field. A 1:1 mixture of soil and vermiculite was
optimal for B. vahlii (Dhar and Upreti 1999), although
Bhatt and Dhar (2000) suggested a 2:1:1
mixture of soilrite, sand and soil.