For each treatment ten replicateswere used. All the treatments were repeated at least three independent times. The treated explants were maintained in a growth chamber at 25 ± 2 °C and 16 hour photoperiod and 8 hour dark period with PPFD of 50 μmol m2/s provided by cool white fluorescent lamps for 15 to 30 days to obtain shoot and root development [22].