15 male albino Wistar rats weighing between 130g-210g were procured from the animal house of the Department of Medical Biochemistry, Cross River University of Technology, Okuku Campus, Cross River State, Nigeria and kept in a clean cage to acclimatize for two weeks. They were monitored under standard laboratory conditions with 12:12 hr light/dark cycle at room temperature (250C ± 2) and fed with standard rat chow and normal water.