Principal exclusion criteria includedhigh-level sporting activities other than tennis and thosenecessary for their training, injury during the three monthsprior to the beginning of the study, diabetes, whethertreated or detected at the inclusion visit, vegetarians andvegans, subjects with extreme eating habits and/or with asignificant history of anorexia nervosa, bulimia or othereating disorders, a history of diseases that could disruptour tests or subjects taking diuretics or other medicationsthat we felt might interfere with our tests, and subjectsconsuming more than two alcoholic beverages per day(every day).