The method presented here not only allows unprecedentedly
high plating efficiency of wall-less D. salina cells,
but also eliminates the need for additional supporting media,
which are common in routine procedures for plating
other wall-less algal cells (Ref. 5). With this successful solidculture
method, single colonies of Dunaliella can be conveniently
obtained and so molecular and physiological studies
on this organism can be carried out on a more-reliable
and -uniform genetic background. In addition, it will surely
accelerate the development of a reliable transformation system
for this organism. Furthermore, screening for mutants with different phenotypes will also be greatly facilitated using
such a solid medium.We believe that the significance of
this method may not be limitated to Dunaliella; with some
modifications, it might also be applied to culturing other wall-less unicellular green algae, or even other unicellular
organisms, on solid media.
The method presented here not only allows unprecedentedlyhigh plating efficiency of wall-less D. salina cells,but also eliminates the need for additional supporting media,which are common in routine procedures for platingother wall-less algal cells (Ref. 5). With this successful solidculturemethod, single colonies of Dunaliella can be convenientlyobtained and so molecular and physiological studieson this organism can be carried out on a more-reliableand -uniform genetic background. In addition, it will surelyaccelerate the development of a reliable transformation systemfor this organism. Furthermore, screening for mutants with different phenotypes will also be greatly facilitated usingsuch a solid medium.We believe that the significance ofthis method may not be limitated to Dunaliella; with somemodifications, it might also be applied to culturing other wall-less unicellular green algae, or even other unicellularorganisms, on solid media.
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