2.9. Preparation of fermented milks
The freeze dried lactic acid bacteria on various supports were
rehydrated in sterile 10% skim milk for 30 min at the end of 60 days
storage. The alginate encapsulated samples were rehydrated with
phosphate buffer at pH 7.Reconstituted skim milk (500 ml) was steamed for 30 min in an
autoclave, cooled to 30 C and inoculated with each of the rehydrated
cells at an inoculum concentration of 10%. The inoculated
milk was incubated at 37 C. The fermentation was monitored for
increase in cell numbers every 4 h, clean coagulation and drop in
pH.