The main effects of the three factors concentration in
sugar cane molasses (2.72), temperature (3.27), and (8.47)
are all positives, and agitation rate is negative (6.66).
The growth time (X4) main effect is the most significant factorial
design effect value for the production of ethanol. The
inclusion of agitation rate reduces the average ethanol production.
Therefore, the subsequent runs were performed in
a static format. Higher ethanol productions, 22.69 g L1
and 30.08 g L1 were obtained in static culture (run 11
and 12 in Table 1). According to Lee and Huang (2000)
Z. mobilis is able to obtain an ethanol production close
to the theoretical one from glucose through Entner–Doudoroff
pathway under aerobic conditions.