The option of increasing overall vitamin B-12 status of deficient population is either fortification or targeted dietary recommendation. The present study measured the effects of supplementation with 600 ml of skimmed buffalo milk not enriched with vitamin B-12, during the day on circulating holo-TC concentration. It also measured the supplementary effects with 400 ml of non-enriched milk daily for 14 days on vitamin B-12 status by measuring the changes in plasma holo-TC, vitamin B-12 and total homocysteine (Hcy) concentrations.