or decoction preparation, each sample (1 g) was
added to 200 mL of distilled water (pH 6.6), heated (heating plate,
VELP scientific, Keyland Court, NY, USA) and boiled for 5 min at
100 C, in a closed recipient to prevent evaporation. The mixture
was left to stand for 5 min and then filtered under reduced pressure
(0.22 mm). The obtained infusions and decoctions were frozen
at 20 C and freeze-dried