Abstract Millettia nitida var. hirsutissima is aChineseherbalmedicineusedforthetreatmentof
gynecological diseases.AnHPLC/DAD/ESI-MSn method wasestablishedfortherapidseparation
and characterizationofbioactiveflavonoidsin M. nitida var. hirsutissima. Atotalof32flavonoids
were detected,ofwhich14compoundswereunambiguouslycharacterizedbycomparingtheir
retention time,UV,andMSspectrawiththoseofthereferencestandards,andtheotherswere
tentatively identifiedbasedontheirtandemmassspectrometryfragmentationdataobtainedinthe
negative ionizationmodeonline.Nineteenofthesecompoundscharacterizedwerereportedfrom
this plantforthefirsttime.
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