The development of portable sensors for rapid, on-site and cost-effective detection of a broad range of targets has long been sought ,because such sensors have the potential to revolutionize scientific research,fast and highly sensitive detection of certain DNA sequences has become increasingly important in the field of drug development,environmental science and and personal healthcare in urban areas in developed countries as well as rural areas in developing countries
Much effort has been made to develop sensitive and selective DNA assay. Among them, traditional techniques, including fluorescence, colorimetry, electrochemistry, surface enhanced Raman scattering (SERS), surface plasmon and light scattering and force, are employed.The detection of limit is low enough for routine detection. However, most of the traditional techniques are expensive, which is not easily available to the public. What is more, sophisticated instrument and operations are required in those methods, which have limited its application.
Therefore, it is still a great challenge to develop portable and quantitative DNA detection method which is easily