2.3. Culture conditions
Rice koji was produced according the method of
Hoppe, Jha, and Egge (1997). Polished rice was steeped
in distilled water for 1 h and steamed at 121 C for 15
min for the purpose of sterilization. A suspension of A.
candidus spores was sprayed onto the surface of the
steamed (cooled) rice (inoculing of 10%, v/w) and incubated
for 15 days.