Five thermotolerant, alcohol-producing yeast cultures were isolated from samples obtained from india. Two were identified as of Kluyveromyces marxianus. All five grew on plate-cultures up to 52 C with maximum growth rates in liquid culture at 40 C. All produced relatively high alcohol concentrations. 5.7 to 7.0%(w/v) at 45 C and 5.0 to 5.5 % (w/v) at 50 C when growing on 14.0% (w/v) glucose. All five isolation fremented diluted molasses containing with P,K,Mg and Mn resulted in a 13 to 20% increase in alcohol production at 40 C. The maximum amounts of alcohol produced on supplemented molasses were 7.5 to 8.0 and 6.5 to 7.0%(w/v) at 37 C and 40 C respectively