Liebermann-Burchard reagent optimization
The samples were prepared as described above, and transferred
to 10 ml volumetric flasks. Different aliquots of reagent LB (1,
2 and 3 ml) were added and the volume was adjusted with
chloroform. After 5 min, the absorbances were measured in
the UV-Vis spectrophotometer, using chloroform to the blank.