After signed informed consent,healthy married men between 30 years and 55 years of age or those with stable chronic medical illnesses,for example,controlled diabetes mellitus and/or hypertension on monotherapy or low dose combination therapy were included (accepted comedication in obese and overweight subject,for example,antidiabetic drug metformin and hypertension, for example, antihypertensive drug amlodipine,atenolol,perindopril).