The dried yeast powder (S. cerevisiae) was aseptically inoculated
into ten 150-ml Erlenmeyer flasks each containing 50 ml sterilized
yeast malt extract (YM) broth. The flasks were incubated at 30 °C for
48 h and 100 rpm in an incubator shaker (Innova 4000, New Brins-
wick Scientific, USA)