Recently, changes in the root protein profile induced by direct Fe deficiency from tomato (Lycopersicon esculentum) [16,17], cucumber (Cucumis sativus) [18], sugar beet (Beta vulgaris) [19] and Medicago truncatula [20] have been investigated by 2-DE proteomic profiling. However, there is little information on the proteomic response of the leaves of Fe-deficient plants. The proteomic profiles of thylakoid membranes of sugar beet [21] and spinach plants [22]