Cell often retains a significant portion of their catalytic activity at the time of nutrient depletion. This catalytically active material discarded upon termination of the fermentation and represents a significant cost in the process. Therefore, production study of laccase on successive repeated batch culture with a selected strain of C. versicolor was carried out with an objective to achieve sustained production of laccase as well as decolorization of dyeing effluent. Repeated batch culture with free mycelium of previous batch in shake flask fermentation for at least 2 times made possible to increase decolorization and laccase production from about 45% to 75% and 10.15 to 39.0 U/ml over 40 days respectively.