If we calculate the antibody binding signals per immobilized
antigens, that is, the protein binding efficiency, and set the MUA
surface to be 100%, then the binding efficiency of the bare gold
surface would be 84%. The best protein binding efficiency of
plasma-treated PS (210%, PS-010-4000, 30 s plasma treatment)
reached 240% of bare gold and 210% of the MUA surface (Fig. 2C).
These data clearly show that PS surfaces with optimal fabrication parameters could significantly enhance the protein binding
response as well as the binding efficiency, although the immobilization density was only slightly improved. Thus, the plasma
treatment ofultrathin PS films was a very suitable approach for the
construction of protein biosensors, as it could immobilize proteins
more readily to their binding pairs.
If we calculate the antibody binding signals per immobilizedantigens, that is, the protein binding efficiency, and set the MUAsurface to be 100%, then the binding efficiency of the bare goldsurface would be 84%. The best protein binding efficiency ofplasma-treated PS (210%, PS-010-4000, 30 s plasma treatment)reached 240% of bare gold and 210% of the MUA surface (Fig. 2C).These data clearly show that PS surfaces with optimal fabrication parameters could significantly enhance the protein bindingresponse as well as the binding efficiency, although the immobilization density was only slightly improved. Thus, the plasmatreatment ofultrathin PS films was a very suitable approach for theconstruction of protein biosensors, as it could immobilize proteinsmore readily to their binding pairs.
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