At first, Anderson medium for shoot induction and multiplication was used. In the experiment, ½ Anderson basal medium with 0.01 mg/l (IAA) were used in total of the treatments. All of the medium in the current work were supplemented with 30 gr sucrose and 7 gr Difco-Bacto agar and the pH was adjusted to 5.8 with 1 N NaOH or 0.1 N HCl prior to autoclaving at 121°c for 30 min.