novel actinomycete, designated strain
NEAU-NH11T, was isolated from muddy soil collected
from a lake and characterized using a polyphasic
approach. The 16S rRNA gene sequence analysis
showed that strain NEAU-NH11T belongs to the genus
Streptosporangium, and was most closely related to
Streptosporangium amethystogenes subsp. amethystogenes
DSM 43179T (99.0 %). Phylogenetic analysis
based on the 16S rRNA gene sequence indicated that
strain NEAU-NH11T formed a monophyletic clade
with Streptosporangium purpuratum CY-15110T
(98.3 %) and Streptosporangium yunnanense CY-
11007T (98.0 %), an association that was supported by
a bootstrap value of 80 %in the neighbour-joining tree
and also recovered with the maximum-likelihood
algorithm. However, the low level of DNA–DNA
relatedness allowed the strain to be differentiated from
S. amethystogenes subsp. amethystogenes DSM
43179T, S. purpuratum CY-15110T and S. yunnanense
CY-11007T. Moreover, strain NEAU-NH11T could
also be differentiated from its closest related strains by
phenotypic characteristics. Therefore, it is proposed
that strain NEAU-NH11T represents a novel Streptosporangium
species, Streptosporangium nanhuense
sp. nov. The type strain of S. nanhuense is NEAUNH11
T. (=CGMCC 4.7131T = DSM 46674T).