The aim of the present study was to determine the prevalence of the main zoonotic bacterial agents transmitted by hematophagous arthropods, in particular A. phagocytophilum, Bartonella spp., B. burgdorferi s.l., C. burnetii and Rickettsia spp., in ticks removed from hunted wild animals living in mountain and hilly areas of Tuscany, central Italy, frequented by visitors for hunting and other recreational activities.