pressure, 2.82 MPa) and agitated at 350 rpm maintained dissolvedoxygen level above 50 % (DO50) for 12.9–19.5 h longerwhen compared with cultures grown in media with a lowerglucose concentration of 40 g L−1 (0.13 MPa) and at sloweragitation rate (175 rpm) (Table S3 in the Electronicsupplementary material; Fig. 5). Greater DO50 values were attributedto increased agitation speed followed by increased glucoseconcentration (Table S3 in the Electronic supplementarymaterial). Increasing agitation speed by itself enhanced dissolvedoxygen levels as well as blastospore production by day3, irrespective of the fungal isolate (F1, 76=94.94, P<0.0001)(Table 3). Interestingly, cultures in media with higher glucoseconcentrations also maintained higher DO levels and supportedbetter yeast-like growth even under low agitation rates for bothisolates (F1, 76=437.15, P<0.0001). There was a positive interactionbetween glucose concentration and agitation speed onblastospore yields (F1, 76=7.58, P=0.0074), which resulted insignificantly higher cell densities from highly aerated and highglucose-amended cultures. Furthermore, it was also noticeablethat more blastospores were produced after 3 days growth whencompared with 2-day-old cultures (F1, 76=84.29, P<0.0001).Significantly greater biomass accumulation was attributed tohigher agitation rates (F1, 78=39.58, P<0.0001), whereas glucoseconcentration alone did not significantly affect biomassaccumulation (F1, 78=0.76, P=0.3849) (Table 3). The finalpH in the culture broth was moderately acidic (4.86±0.2 forESALQ1432 and 5.22±0.14 for GHA) and was similar for allcultures (data not shown
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