The equation of a typical calibration line was A=0.0017
+ 0.0024 with a correlation coefficient (R2=0.989), where A is the
absorbance and C is the copper concentration in solution (mg L1).
The enhancement factor (EF) calculated as the ratio of the slope of
calibration curve of the copper(II) after preconcentration to that of
prior preconcentration was found as 53.The limit of detection,
LOD, was calculated as the ratio of three times standard deviation
of ten blank absorbances to the slope of the calibration curve was
0.52 mg L1, where the limit of quantification, LOQ,was found as
the ratio of ten times the standard deviation of the ten blank
solution to the slope of the calibration curve was1.71 mg L1. The
precision of the presented method was given as relative standard
deviation (RSD) which evaluated for seven successive supramolecular solvent microextraction of 25 mg L1 of copper(II) was 2.6%.